detecting the p24 antigen in lymph nodes of blv infected cattle

نویسندگان

غلامرضا نیکبخت بروجنی

گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران فرهید همت زاده

گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران سید مهدی امام

گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران مهدی غفاری

گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران

چکیده

in this study 19 blv infected and 2 healthy lymph nodes were analysed by western blotting to detect viral p 24 antigen . western blotting was carried out by rabbit anti p24 serum and p24 monoclonal antibody. rabbit serum in western blotting could detect 45, 50, 54, 58 and less than 19 kd bands of proteins and monoclonal antibody could detect 30, 32, 39, 45, 50 and 53 kd bands. our results indicate polymorphism of proteins with p24 epitope in different blv infected samples. otherwise, it could be concluded that blv structural proteins sharing p24 epitopes have high degree of phenotypic diversity. in this study 19 blv infected and 2 healthy lymph nodes were analysed by western blotting to detect viral p 24 antigen . western blotting was carried out by rabbit anti p24 serum and p24 monoclonal antibody. rabbit serum in western blotting could detect 45, 50, 54, 58 and less than 19 kd bands of proteins and monoclonal antibody could detect 30, 32, 39, 45, 50 and 53 kd bands. our results indicate polymorphism of proteins with p24 epitope in different blv infected samples. otherwise, it could be concluded that blv structural proteins sharing p24 epitopes have high degree of phenotypic diversity.

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تحقیقات دامپزشکی

جلد ۶۲، شماره ۳، صفحات ۲۸۵-۲۸۹

کلمات کلیدی

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